We are interested to understand how trypanosomes sense their environment and how they regulate gene expression in order to adapt to different hosts, with particular emphasis on genes coding for stage-specific coat proteins. Many other organisms regulate expression primarily at the level of the promoter. In contrast, trypanosomes typically employ multiple levels of post-transcriptional control. We are using several approaches, including biochemical purification of RNA-binding complexes and genome-wide screens with RNAi libraries, to identify novel proteins that regulate stage-specific gene expression. One of our aims is to identify classes of mRNAs that are coordinately regulated by specific ribonucleoprotein complexes and to decipher the sequence and structural motifs that determine binding..
Trypanosome, gene expression, RNA-binding, post-transcriptional control, surface glycoprotein