Models of the hexameric cleavage stimulation factor (CstF), which is involved in 3′ end processing of vertebrate mRNAs. CstF is normally composed of two subunits each of CstF-77 (yellow), CstF-64 (green), and CstF-50 (blue), but a CstF-64 paralogue called CstF-64Tau (red) is also expressed ubiquitously. When the concentration of CstF-64 becomes limiting, CstF-64Tau is incorporated into CstF complexes even though its affi nity for CstF-77 is lower than that of CstF-64. As a consequence, CstF-64Tau is up-regulated by protein stabilization. Because the CstF-64 subunits are responsible for binding to GU-rich sequences in pre-mRNA substrates, this regulation may lead to changes in poly(A) site selection (Ruepp et al. MBoC 2011). The models were assembled with Lego Digital Designer 3.1 software and graphically edited in Photoshop CS. (Image: Nicole Kleinschmidt, University of Berne, Switzerland)
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Key publications of Schümperli group
Original articles
- Ruepp, M.-D., Schweingruber, C., Kleinschmidt, N. and Schümperli, D. (2011) Interactions of CstF-64, CstF-77, and symplekin: Implications on localisation and function. Mol. Biol. Cell 22, 91-104. Epub 2010 Nov 30
- Ruepp, M.-D., Vivarelli, S., Pillai, R.S., Kleinschmidt, N., Azzouz, T.N., Barabino, S.M.L. and Schümperli D. (2010) The 68 kDa subunit of mammalian cleavage factor I interacts with the U7 small nuclear ribonucleoprotein and participates in 3' end processing of animal histone mRNAs. Nucl. Acids Res. 38, 7611-7625. Epub 2010 Jul 15.
- Meyer, K., Marquis, J., Trüb, J., Nlend Nlend, R., Verp, S., Ruepp, M.-D., Imboden, H., Barde, I., Trono, D. and Schümperli, D. (2009) Rescue of a severe mouse model for Spinal Muscular Atrophy by U7 snRNA-mediated splicing modulation. Hum. Mol. Genet. 18, 546-555. Epub 2008 Nov 13.
- Pillai, R.S., Grimmler, M., Meister, G., Will, C.L., Lührmann, R., Fischer, U. and Schümperli, D. (2003) Unique Sm core structure of U7 snRNPs: assembly by a specialised SMN complex and the role of a new component, Lsm11, in histone RNA processing. Genes Dev. 17, 2321-2333.
- Pillai, R.S., Will, C.L., Lührmann, R., Schümperli, D. and Müller, B. (2001) Purified U7 snRNPs lack the Sm proteins D1 and D2 but contain Lsm10, a new 14 kDa Sm D1-like protein. EMBO J. 20, 5470-5479.
Reviews
- Nlend Nlend, R., Meyer, K. and Schümperli, D. (2010) Repair of pre-mRNA splicing: Prospects for a therapy for Spinal Muscular Atrophy. RNA Biology 7, 730-740, published online May 28.
- Ruepp, M.-D., Barabino, S.M.L. and Schümperli D. (2010) mRNA 3' end processing and more - multiple functions of mammalian cleavage factor I-68. WIREs RNA 2, 79-91, published online July 15, DOI:10.1002/wrna.35.
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