Prof. Dr. Beat Suter

Controlling development by localizing mRNAs and organelles

A large proportion of mRNAs, proteins and organelles needs to become localized to specific and divers cellular compartments in order to polarize cells, to direct cellular development, and to efficiently focus the expression of proteins to specific regions of cells. Targeted delivery of mRNAs also provides complex cells with the opportunity to rapidly, locally and temporally control gene expression through local induction of translation, for instance through a local signal. Surprisingly, a very small number of cellular transport systems are responsible for generating a larger number of diverse localization patterns. Particularly transport towards the minus ends of microtubules depends almost exclusively on the cytoplasmic dynein motor. Coupling different cargo adaptors and using different gears and a clutch provides solutions for the required activities. We study aspects of the dynein / dynactin / BicD-mediated localization process to learn more about how this molecular motor localizes his amazing variety of cargos.

Aside from the basic science aspect there is also a human disease aspects to this research. Components of this localization machinery have been linked to human diseases. Lis1 (Lissencephaly-1) plays a crucial role in brain development and mutations in the human BICD2 gene cause Spinal Muscular Atrophy
and also defects in brain development. The Drosophila model allows us to study these genes utilizing a variety of experimental approaches.

Selected primary research articles:
Vazquez-Pianzola et al., RNA Biology, doi: 10.1080/15476286.2016.1251542.
Vazquez-Pianzola et al., Development 141, 1915-1926. doi: 10.1242/dev.099432
Dix et al., J. Cell Biol. 202, 479–494.
Vazquez-Pianzola et al., Dev. Biol. 357, 404-418. doi:10.1016/j.ydbio.2011.07.009
Koch et al., PLoS ONE 4(2): e4552.
Cavey et al., Development 132, 459-468.
Houalla et al., Mech. Dev. 122, 97-108.
Paré and Suter, J Cell Sci. 113, 2119-2127.
Swan et al., Nature Cell Biology 1, p444-449.
Stuurman et al., Eur. J. Cell Biol. 78, 278-287.
Swan and Suter, Development 122, 3577-3586.
Ran et al., Development 120, 1233-1242.
Suter and Steward, Cell 67, 917-926.
Suter et al., Genes & Dev. 3, 1957-1968.

Spin-off projects
Börner et al., Developmental Biology 411, 217–230.
Murakami et al., Current Biology 26, 972-980.
Urwyler et al., Biology Open, doi: 10.1242/bio.20122154.
Claußen et al., Genetics 174, 1337–1347.

Cell cycle control: Cdk7 and Xpd

The protein kinase Cdk7 is an essential activator of the mitotic cell cycle kinase Cdk1. However, it also participates in other physiological activities, particularly in transcription. These two processes are mutually exclusive and Cdk7 switches from one function to the other during the cell cycle. We found that Xpd plays a role in directing the Cdk7 activity towards the correct target during the cell cycle. Indeed, Xpd can be found in complexes with Cdk7 and it appears to act as a dispatcher that sends the Cdk7 kinase towards specific cellular substrates or prevents it from targeting a substrate class at particular phases of the cell cycle. In this way Xpd negatively regulates the cell cycle function of Cdk7, the phosphorylation of Cdks, and it recruits Cdk7 into the TFIIH complex where it functions in transcription. This attributes an apparently direct cell cycle function to Xpd, in addition to its roles in DNA repair and transcription.

Studying the cell cycle function of Xpd we found that Xpd is essential for the proper dynamics of the mitotic spindle and for segregation of the chromosomes at the exit from mitosis. Lack of Xpd and mutations in xpd cause chromosomal instability (CIN), a hallmark of cancer. Furthermore, Drosophila Xpd coordinates mitotic cycles between thousands of embryonic nuclei that share a common cytoplasm. Using Drosophila as experimental model system, we were able to show how mutations identified in human XPD patients affect the function of Xpd and how this might contribute to the human XPD phenotypes. Why does Xpd control Cdk7 only during specific phases of the cell cycle? Who regulates Xpd? These are the questions we are presently addressing.

Selected primary research articles:
Stettler et al., Disease Models & Mechanisms 8, 81-91 doi:10.1242/dmm.016907.
Cameroni et al., Cell Division, 5, 24
Li et al., PLoS Genet 6, e1000876.
Schwartz et al., Mol. Cell. Biol. 23, 6876-6886.
Chen and Suter Cell Cycle 2; 503-506.
Chen et al., Nature 424: 228-232.
Masrouha et al., Genetics 163: 973–982.
Larochelle et al., EMBO J. 20; 3749-3759.
Larochelle et al., Genes & Dev. 12, 370-381.

PheRS and multifunctional proteins

Aminoacyl-tRNA synthetases (aaRSs) ligate amino acids to their cognate tRNAs, allowing them to decode the triplet code during translation. For accurate decoding it is imperative that the enzyme recognizes the appropriate amino acid and that it charges the proper tRNA with the correct amino acid. The enzyme that charges tRNAPhe with the amino acid Phe, PheRS, uses a 2-step process to achieve a high decoding accuracy. A first sieve is an amino acid binding pocket that is too small for the similar amino acid Tyr. In addition, there is a proof reading mechanism that can sieve out the wrongfully activated or charged Tyr.

Mutations in many different human aaRSs cause human diseases (particularly neurodegenerative diseases) and aaRSs have also been linked to signaling and cancer. Interestingly, many aaRSs also perform additional functions in the cell, unrelated to their aminoacylation function. It is therefore not known how these aaRS mutations lead to the disease. In order to assess the importance of aminoacylation fidelity in multicellular organisms, we generated an amino-acid double-sieving model in Drosophila melanogaster using phenylalanyl-tRNA synthetase (PheRS). Double-sieving-defective mutations dramatically misacylate non-cognate Tyr, induce protein mistranslation and cause endoplasmic reticulum stress in flies. Mutant adults exhibit severe defects, including loss of neuronal cells, impaired locomotive performance, shortened lifespan and smaller organ size. The mutations also reduce cell proliferation and promote cell death. We are now trying to identify the non-canonical functions of Drosophila PheRS.

Lu et al., Nature Commun 5:5650 | DOI: 10.1038/ncomms6650.
Lu et al., Fly, 9, 53-61, DOI: 10.1080
Dolde et al., Advances in Biology, Volume 2014, Article ID 274196